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Invitrogen™ Digitonin (5%)
Digitonin (5%), or digitin, is a non-ionic detergent with a cholesterol-like structure that can water-solubilize lipids, permeabilize cells, be used as a cytotoxicity positive control and solubilize membrane-bound proteins for NativePAGE™ protein analysis or cryo-EM structural analysis.
Merk: Invitrogen™ BN2006
Inclusief: 1 mL of solution
Beschrijving
Digitonin, also known as digitin, is a mild, non-ionic detergent with a cholesterol-like structure that allows it to water-solubilize lipids. It is useful for permeabilizing cells and can be used as a cytotoxicity chemistry positive control. In addition, it preserves the native lipid environment in membrane protein complexes and can be used to solubilize membrane-bound proteins. Digitonin has a molecular formula of C56H92O29 and molecular weight of 1229 Daltons.
Digitonin detergent solution has many more applications. It is used to prepare native protein samples for analysis on the NativePAGE Gel System. Included in the NativePAGE Sample Prep Kit (Cat No. BN2008), this ready-to-use detergent solution improves solubility of hydrophobic and membrane proteins during sample preparation. Samples prepared with 10% DDM, 5% digitonin, or the NativePAGE Sample Prep Kit are compatible with NativePAGE Novex Bis-Tris Gels for native electrophoresis and have increased resolution and reduced streaking. In addition, before structural determination by cryo-EM or crystallization, digitonin can be used to solubilize membrane proteins (typically at 1–2% w/v) and as a wash buffer.
It is important to note for sample preparation that the digitonin detergent micelle size is ∼70 kDa and is typically used at a concentration that is 1–2X the critical micelle concentration (CMC).
Furthermore, digitonin can be used as a spike in purified protein at CMC concentration to help with particle distribution and preferred orientation on grid during the vitrification process. In addition, digitonin provides efficient antibody penetration into tissue sections and is compatible with immunohistochemistry (IHC) staining. Digitonin at a final concentration of 0.2–0.5% in buffer is compatible with IHC.
NOTE: If the 5% digitonin solution precipitates out of solution, warm the solution at 37°C and gently shake to dissolve the precipitate.
Informatie over bestelling
Shipping Condition: Wet Ice
Specificatie
| 11024-24-1 | |
| Clear | |
| IHC, Cryo-EM, Crystallography | |
| Native-PAGE | |
| 1 mL | |
| Native Gel Electrophoresis | |
| 1229 | |
| Digitonin |
| Store at 2–8°C. | |
| 5% | |
| Mini Gel Tank, XCell SureLock Mini-Cell | |
| Digitonin Solution | |
| Liquid | |
| NativePAGE Gels | |
| 1 mL | |
| Wet Ice |
Veiligheid en behandeling
Productidentificatie
Signaalwoord
Gevarencategorie
Gevarenaanduiding
Veiligheidsmaatregel
Aanvullende informatie
- Digitonin (5%)
Signaalwoord
- Waarschuwing
Gevarencategorie
- Acute toxiciteit Categorie 4
- Dzia anie toksyczne na narzady docelowe Categorie 2
Gevarenaanduiding
- H302-Schadelijk bij inslikken.
- H373-Kan schade aan organen.
Veiligheidsmaatregel
- P260-Stof/rook/gas/nevel/damp/spuitnevel niet inademen.
- P264-Na het werken met dit product grondig wassen.
- P270-Niet eten, drinken of roken tijdens het gebruik van dit product.
- P301+P310-NA INSLIKKEN: onmiddellijk een ANTIGIFCENTRUM/arts/ raadplegen.
- P314-Bij onwel voelen een arts raadplegen.
- P330-De mond spoelen.
Aanvullende informatie
- MIXTURE LIST-Bevat: digitonin
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Product Title
For Research Use Only. Not for use in diagnostic procedures.
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