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  6. Thermo Scientific™ NE-PER™ Nuclear and Cytoplasmic Extraction Reagents

Thermo Scientific™ NE-PER™ Nuclear and Cytoplasmic Extraction Reagents

Efficiently lyse cells and extract separate cytoplasmic and nuclear protein fractions in less than two hours with this subcellular fractionation kit.

400.00 - 748.00

Specificatie

Producttype Nuclear and Cytoplasmic Extraction Reagent
Shipping Condition Ambient
Vorm Liquid
Product Line NE-PER™
Buffer Lysis Buffer
Meer specificaties weergeven

Producten 2
Produktcode Merk Aantal Prijs Hoeveelheid en beschikbaarheid  
Produktcode Merk Aantal Prijs Hoeveelheid en beschikbaarheid  
10309383
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Thermo Scientific™
78835 		Kit van 75 mL
748.00
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11865694
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Thermo Scientific™
78833 		Kit van 15 mL
400.00
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Beschrijving

Beschrijving

Thermo Scientific™ NE-PER Nuclear and Cytoplasmic Extraction Kit provides for efficient cell lysis and extraction of separate cytoplasmic and nuclear protein fractions in less than two hours.

The NE-PER Kit is a nuclear protein extraction method that involves simple, stepwise lysis of cells and centrifugal isolation of nuclear and cytoplasmic protein fractions. A benchtop microcentrifuge, tubes and pipettors are the only tools required. The NE-PER Reagents efficiently solubilize and separate cytoplasmic and nuclear proteins into fractions with minimal cross-contamination or interference from genomic DNA and mRNA. Once desalted or diluted, the isolated proteins can be used to perform immunoassays and protein interaction experiments, such as mobility shift assays (EMSA), co-immunoprecipitation (Co-IP) and pull-down assays.

Highlights:

  • Fast – obtain nuclear and cytoplasmic fractions in less than two hours
  • Proven – the NE-PER Kit is referenced in more than 950 distinct publications
  • Scalable – two kit sizes for producing extracts from cells and tissues
  • Convenient – simple instructions do not require ultracentrifugation over gradients
  • Compatible – use for downstream assays, including Western blotting, gel-shift assays, protein assays, reporter gene assays and enzyme activity assays

Includes:

Two kit sizes, each containing three buffer and detergent solutions

Compatible with:

Thermo Scientific LightShift Chemiluminescent EMSA Kit (Mfr. No. 20148)

Storage Requirements:

Upon receipt store kit components at 4°C

Specificatie

Specificatie

Nuclear and Cytoplasmic Extraction Reagent
Liquid
Lysis Buffer
Ambient
NE-PER™
Cell Lysis Buffer, Detergent Solution, Subcellular Fractionation
Video's
Veiligheid en behandeling

Veiligheid en behandeling

Productidentificatie
  • NE-PER Nuclear and Cytoplasmic Extraction Reagents

Signaalwoord
  • Waarschuwing

Gevarencategorie
  • Ernstig oogletsel/oogirritatie Categorie 2
  • Huidcorrosie/-irritatie Indeling Categorie 2

Gevarenaanduiding
  • H315-Veroorzaakt huidirritatie.
  • H319-Veroorzaakt ernstige oogirritatie.

Veiligheidsmaatregel
  • P264-Na het werken met dit product grondig wassen.
  • P280-Beschermende handschoenen/beschermende kleding/oog­bescherming/gelaatsbescherming dragen.
  • P302+P352-BIJ CONTACT MET DE HUID: met veel water/ wassen.
  • P305+P351+P338-BIJ CONTACT MET DE OGEN: voorzichtig afspoelen met water gedurende een aantal minuten; contactlenzen verwijderen, indien mogelijk; blijven spoelen.
  • P332+P313-Bij huidirritatie: een arts raadplegen.
  • P337+P313-Bij aanhoudende oogirritatie: een arts raadplegen.
  • P362+P364-Verontreinigde kleding uittrekken en wassen alvorens deze opnieuw te gebruiken.

Aanvullende informatie
  • MIXTURE LIST-Bevat: non ionic detergent

SDS
Documentatie

Documentatie

Product Certifications

For Research Use Only. Not for use in diagnostic procedures.

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