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  5. Invitrogen™ PureLink™ Genomic DNA Mini Kit
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Invitrogen™ PureLink™ Genomic DNA Mini Kit

The PureLink™ Genomic DNA Mini Kit enables high-yield, high-purity, genomic DNA (gDNA) extractions from a wide variety of sample types. Use this single kit for genomic DNA purification from blood, tissues, cells, bacteria, swabs, and blood spots, with a familiar silica-based, microcentrifuge spin-column format.

Merk:  Invitrogen™ K182002


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Produktcode. 10458843

  • 968.00 /

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Beschrijving

Beschrijving

The PureLink™ Genomic DNA Mini Kit enables high-yield, high-purity, genomic DNA (gDNA) extractions from a wide variety of sample types. Use this single kit for genomic DNA purification from blood, tissues, cells, bacteria, swabs, and blood spots, with a familiar silica-based, microcentrifuge spin-column format. Features of the PureLink™ Genomic DNA Mini Kit include:

• Kit flexibility—one kit works with a variety of sample types and sizes
• Ultraclean gDNA—minimal contamination of DNA product means successful downstream application
• Improved design—optimized spin-column design and buffer formulation for better yield and purity

Multiple sample sources, one kit
The PureLink™ Genomic DNA Mini Kit can be used with many different sample types, each with its own optimized protocol outlined in the manual.

Optimized components and protocols
The PureLink™ Genomic DNA Mini Kit includes parts and protocols that improve upon the easy and familiar spin-column methods currently in use. Columns are newly configured to bind and release DNA more efficiently. Each sample source uses a specialized lysis procedure and an optimized lysis buffer formulated to enhance proteinase K activity and eliminate protein contamination. A chaotropic salt buffer promotes stable binding of DNA to the column resin, and powerful wash buffers remove all traces of protein and salt. DNA is eluted in a low-salt buffer to allow for pH stabilization of the DNA in storage.

Higher yields and purity
With PureLink™ Genomic DNA Kits you can expect high yields of high-purity gDNA (determined by A260/A280 measurements), no matter the sample type, from bacteria to tissues to blood to cultured cells. The enhanced PureLink™ Lysis and Wash Buffers help to consistently deliver purified gDNA samples with absorbance reading ratios of ∼1.9 for both A260/A280 and A260/A230, indicating no detectable contamination from carryover of protein or guanidine.

Informatie over bestelling

Shipping Condition: Room Temperature

TRUSTED_SUSTAINABILITY
Specificatie

Specificatie

• 50 mL PureLink Genomic Lysis/Binding Buffer; room temperature
• 45 mL PureLink Genomic Digestion Buffer; room temperature
• 50 mL PureLinkGenomic Wash Buffer 1; room temperature
• 37.5 mL PureLink Genomic Wash Buffer 2; room temperature
• 50 mL PureLink Genomic Elution Buffer; room temperature
• 5 mL RNase A (20 mg/mL); room temperature
• 5 mL Proteinase K (20 mg/mL); room temperature, or 4°C for long-term storage
• 5 x 50 PureLink Spin Columns with Collection Tubes; room temperature
• 5 x 100 PureLink Collection Tubes; room temperature
Mini
25 to 200 μL
PCR, Southern blotting, sequencing, nucleic acid labeling, hybridization
250 Preps
Up to 15 min. after cell lysis
Bacteria: ≤109 cells
Cells: ≤106
Tissue: ≤25 mg
Mouse Tail: 1 cm
Rat Tail: 0.5 cm
Whole Blood: ≤1 mL
Yeast: ≤107 cells
FFPE: ≤8 sections of 5-15 μM thickness
Oragene™ Preserved Saliva: ≤4 mL
Blood Spots: ≤5 punches
Silica Spin Column
Bacteria, Cells, Dried Blood Spots, FFPE (Formalin-fixed paraffin-embedded), Mouse/Rat Tail, Saliva, Buccal Swab, Tissue, Whole Blood, Yeast
gDNA
Not High-throughput Compatible (Manual)
Room Temperature
≤40 μg
Product Suggestions

Product Suggestions

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Veiligheid en behandeling

Veiligheid en behandeling

Productidentificatie
  • PURELINK GENOMIC DNA KIT (250)

Signaalwoord
  • Gevaar

Gevarencategorie
  • Acute toxiciteit Categorie 4
  • Ernstig oogletsel/oogirritatie Categorie 2
  • Sensibilisatie van de luchtwegen Categorie 1
  • Huidcorrosie/-irritatie Indeling Categorie 2
  • Sensibilisering van de huid Categorie 1

Gevarenaanduiding
  • H302-Schadelijk bij inslikken.
  • H315-Veroorzaakt huidirritatie.
  • H317-Kan een allergische huidreactie veroorzaken.
  • H319-Veroorzaakt ernstige oogirritatie.
  • H332-Schadelijk bij inademing.
  • H334-Kan bij inademing allergie- of astmasymptomen of ademhalingsmoeilijkheden veroorzaken.

Veiligheidsmaatregel
  • P261-Inademing van stof/rook/gas/nevel/damp/spuitnevel vermijden.
  • P264-Na het werken met dit product grondig wassen.
  • P270-Niet eten, drinken of roken tijdens het gebruik van dit product.
  • P272-Verontreinigde werkkleding mag de werkruimte niet verlaten.
  • P280-Beschermende handschoenen/beschermende kleding/oog­bescherming/gelaatsbescherming dragen.
  • P284-¢Bij ontoereikende ventilatie! adembescherming dragen.
  • P301+P310-NA INSLIKKEN: onmiddellijk een ANTIGIFCENTRUM/arts/ raadplegen.
  • P302+P352-BIJ CONTACT MET DE HUID: met veel water/ wassen.
  • P304+P340-NA INADEMING: de persoon in de frisse lucht brengen en ervoor zorgen dat deze gemakkelijk kan ademen.
  • P305+P351+P338-BIJ CONTACT MET DE OGEN: voorzichtig afspoelen met water gedurende een aantal minuten; contactlenzen verwijderen, indien mogelijk; blijven spoelen.
  • P330-De mond spoelen.
  • P332+P313-Bij huidirritatie: een arts raadplegen.
  • P333+P313-Bij huidirritatie of uitslag: een arts raadplegen.
  • P337+P313-Bij aanhoudende oogirritatie: een arts raadplegen.
  • P342+P311-Bij ademhalingssymptomen: Een ANTIGIFCENTRUM/arts/ raadplegen.
  • P362+P364-Verontreinigde kleding uittrekken en wassen alvorens deze opnieuw te gebruiken.
  • P501b-Inhoud/verpakking afvoeren naar verwerken volgens de plaatselijke/regionale/nationale/internationale voorschriften.

Aanvullende informatie
  • MIXTURE LIST-Bevat: Guanidine hydrochloride, Proteinase, tritirachium album serine

SDS
Documentatie

Documentatie

Certificates
Speciale aanbiedingen

Speciale aanbiedingen

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