missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Biochemical Reagents
  4. Nucleic Acid Introduction Reagents
  5. Transfection Reagents
  6. 10124462

Invitrogen™ Calcium Phosphate Transfection Kit

Provides high-quality reagents to enable the introduction of DNA into eukaryotic cells via calcium phosphate co-precipitation

Merk:  Invitrogen™ K278001

642.20 EUR valid until 2025-12-31
Use promo code "24270" to get your promotional price.

Produktcode. 10124462

  • 676.00 /

Please to purchase this item. Need a web account? Register with us today!

Explore more special offers

Inclusief: 2 x 12mL Tissue Culture Sterile Water, 2 x 12mL 2X HEPES Buffered Saline (HBS), 3 x 1mL 2M CaCl2, 20μg pcDNA™3.1/His/lacZ
This item is not returnable. View return policy

Beschrijving

Beschrijving

  • Based on forming a calcium phosphate-DNA precipitate
  • Facilitates the binding of the DNA to the cell surface. DNA then enters the cell by endocytosis
  • First developed by Graham and van der Ebb and was later modified by Wigle
  • Used to transfect a wide variety of cell types for transient expression or for producing stable transformants
  • DNA is mixed directly with a concentrated solution of CaCl2, which is then added dropwise to a phosphate buffer to form a fine precipitate
  • Aeration of the phosphate buffer while adding the DNA-CaCl2 solution helps to ensure that the precipitate that forms is as fine as possible, which is important because clumped DNA will not adhere to or enter the cell as efficiently
  • Product size: 75 reactions

Cell Culture, Plasmid Transfection, Transfection

Informatie over bestelling

Shipping Condition: Room Temperature

TRUSTED_SUSTAINABILITY
Specificatie

Specificatie

2 × 12 ml Tissue Culture Sterile Water
2 × 12 ml 2X HEPES Buffered Saline (HBS)
3 × 1 ml 2 M CaCl2
20 μg pcDNA™3.1/His/lacZ

Store all components at -5 to -30°C.
6-well Plate, 12-well Plate, 24-well Plate, 48-well Plate, 96-well Plate, Flasks
Chemical Transfection
Not High-throughput Compatible (Manual)
75 Reactions
Established Cell Lines
Plasmid DNA
Transfection
Transfection Kit
No
Product Suggestions

Product Suggestions

Video's
SDS
Documentatie

Documentatie

Certificates
Speciale aanbiedingen

Speciale aanbiedingen

Product Content Correction

Your input is important to us. Please complete this form to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Thank You! Your feedback has been submitted. Fisher Scientific is always working to improve our content for you. We appreciate your feedback.

For Research Use Only. Not for use in diagnostic procedures.