Invitrogen™ AccuPrime™ Pfx DNA Polymerase

Beschrijving

AccuPrime Pfx DNA Polymerase is ideal for high-fidelity amplification of DNA fragments for downstream applications such as cloning and mutagenesis. High fidelity is provided by a proprietary enzyme preparation containing recombinant DNA polymerase from Thermococcus species KOD with proofreading (3'→5' exonuclease) activity. Platinum anti-Pfx DNA polymerase antibodies inhibit polymerase activity, providing hot-start capabilities for improved PCR specificity (see figure). Thermostable AccuPrime accessory proteins enhance specific primer-template hybridization during every cycle of PCR, increasing specificity, yield, and robustness over Platinum Pfx alone.

Benefits of AccuPrime Pfx DNA Polymerase

• Greater than 26 times higher fidelity than Taq DNA polymerase
• Amplification of fragments up to 12 kb
• Minimized PCR optimization

Applications

• Amplification of DNA from complex genomic, viral, and plasmid templates
• RT-PCR
• High-fidelity PCR
• Hot-start PCR

Notes

• AccuPrime Pfx DNA Polymerase is especially effective where very high levels of fidelity are required.
• The 10X AccuPrime Pfx reaction mix contains MgSO₄, dNTPs, and thermostable proteins that enhance specific primer-template hybridization during each PCR cycle.

Informatie over bestelling

Approved for shipment on Wet or Dry Ice