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Cytiva Single Cell GenomiPhi™ DNA Amplification Kit
Vermeerder DNA van het hele genoom uit slechts één cel. GE Healthcare Lifescience™ illustra™ Single Cell GenomiPhi™ DNA-amplificatiekit verenigt de unieke capaciteiten van Phi29 DNA-polymerase met een geoptimaliseerde samenstelling voor het vermeerderen van genoom-DNA uit 1-1000 cellen zonder achtergrondstoring.
Merk: Cytiva 29108039
Inclusief: Eencellige GenomiPhi-reactiebuffer 1 x 275 µl, eencellig GenomiPhi-enzymmengsel 1 x 25 µl, eencellig GenomiPhi-vermeerderingsmengsel 1 x 25 µl, eencellige GenomiPhi DTT-oplossing 1 x 100 µl, eencellige GenomiPhi-lysisbuffer 1 x 100 µl en eencellige GenomiPhi-neutralisatiebuffer 1 x 100 µl
Beschrijving
- For whole genome amplification of genomic DNA from as little as a single cell
- Novel clean-up process supresses background allowing sensitivity down to 1fg of input DNA
- Simple Isothernal strand displacement (MDA) technology using Phi29 DNA polymerase
- Amplifications can be completed in less than 3 hours with less than 15 minutes hands-on time
- Optimized to promote complete cell lysis with uniform denaturation of DNA prior to amplification
- Reaction time: 2 to 4 hours
- Product average size: >10kb
For SNP arrays, Next Gen Sequencing, aCGH
Recommended StorageStore the kit at -70°C. The enzyme mix must be stored at -70°C; all other components may be stored at -20°C
Specificatie
| Store the kit at -70°C. The enzyme mix must be stored at -70°C; all other components may be stored at -20°C. | |
| illustra™ Single Cell GenomiPhi™ DNA Amplification Kit | |
| Single Cell GenomiPhi Reaction buffer 1 x 275μL, Single Cell GenomiPhi Enzyme mix 1 x 25μL, Single Cell GenomiPhi Amplification mix 1 x 25μL, Single Cell GenomiPhi DTT Solution 1 x 100μL, Single Cell GenomiPhi Lysis buffer 1 x 100μL and Single Cell GenomiPhi Neutralisation buffer 1 x 100μL | |
| illustra Single Cell GenomiPhi DNA Amplification Kit | |
| 2 to 4 hours |
| Single Cell GenomiPhi DNA Amplification kit combines the unique capabilities of Phi29 DNA polymerase with an optimized formulation to amplify genomic DNA from 1-1000 cells without background interference. | |
| Genomic DNA Amplification | |
| 100 | |
| >10kb |
Veiligheid en behandeling
Signaalwoord
Gevarencategorie
- Gevaar
Gevarencategorie
- Huidcorrosie/-irritatie Indeling Categorie 1
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