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Cytiva HiTrap™ TALON™ Crude Prepacked Columns

For purification of histidine-tagged recombinant proteins by immobilized metal affinity chromatography (IMAC). Cytiva Lifescience™ HiTrap™ TALON™ Crude Prepacked Columns offer a cobalt-based medium allowing protein purification under native or denaturing conditions.

Merk:  Cytiva 29048565


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Produktcode. 15147946

  • 59.00 / 1 ml
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Beschrijving

Beschrijving

Cobalt-based IMAC medium offering different selectivity compared to nickel-charged media. Allows protein purification under native or denaturing conditions and can be used with prokaryotic and eukaryotic expression systems.

  • Prepacked with cobalt-based chromatography media
  • Binding capacity approximately 7mg MBP
  • Maximum pressure: 3 bar
  • Column material: polypropylene
  • Compatible with commonly used buffers and easily regenerated with 0.5 M NaOH
  • 1 and 5mL columns

Media
  • Ligand: TALON™ Superflow Cobalt-IMAC
  • Matrix: 6% cross-linked agarose
  • Average particle size: 60 to 160μm
  • pH stability working range: 3 to 12

1mL columns

  • Column I.D.: 7mm
  • Flow velocity: 1 to 4mL/min.

5mL columns
  • Column I.D.: 16
  • Flow velocity: 5 to 20mL/min.

TRUSTED_SUSTAINABILITY
Specificatie

Specificatie

1 x 1 mL
4°C to 8°C
0.27 in.
0.7 cm
60 to 160 μm
3 bar
3 to 12
Talon Superflow Cobalt-IMAC
For PurifiCatIon of Histidine-Tagged Recombinant Proteins by Immobilized Metal Affinity Chromatography, Used Buffers and Easily Regenerated with 0.5 M NaOH
Up to 20 mg Histidine-tagged Protein1
HiTrap Talon crude prepacked Column
1 to 4 mL/min
Polypropylene
6% Cross-Linked Agarose
20% Ethanol
1 x 1 mL
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  • Waarschuwing

Gevarencategorie
  • Ontvlambare vloeistof Categorie 3

Gevarenaanduiding
  • H226-Ontvlambare vloeistof en damp.

Veiligheidsmaatregel
  • P210-Verwijderd houden van warmte, hete oppervlakken, vonken, open vuur en andere ontstekingsbronnen. Niet roken.
  • P241-Explosieveilige elektrische/ventilatie-/verlichtings-/ apparatuur gebruiken.
  • P501b-Inhoud/verpakking afvoeren naar verwerken volgens de plaatselijke/regionale/nationale/internationale voorschriften.

Aanvullende informatie
  • MIXTURE LIST-Bevat: ethanol, methanol, cobalt

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