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Cytiva HisTrap™ FF Columns: 16 x 25mm
Use for optimized purification of histidine-tagged proteins directly from homogenized, unclarified cells by immobilized metal ion affinity chromatography (IMAC). Cytiva Life Sciences™ HisTrap™ FF Columns: 16 x 25mm is a ready-to-use column, prepacked with precharged Ni Sepharose™ 6 Fast Flow.
Merk: Cytiva 17-5319-02
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Beschrijving
- Prepacked with precharged Ni Sepharose™ 6 Fast Flow media
- Column material: polypropylene
- Bed dimensions: 16 × 25mm
- Flow velocity: <20mL/min.
- Protein binding capacity of ∽40mg/mL media with negligible nickel ion leakage
- Compatible with wide range of reducing agents, detergents, denaturants, and other additives
- Simple operation with syringe, perstaltic pump, or chromatographic system
- Columns may be connected in series to increase capacity
Medium
- Matrix: 6% cross-linked agarose
- Average particle size: 90μm
- pH stability working range: 3 to 12
Specificatie
| 5 mL | |
| 16 mm | |
| <20 mL/min. | |
| 3 to 12 | |
| 4°C to 30°C, 20% Ethanol | |
| 100 x 1 mL |
| 0.6 in. | |
| HisTrap FF is a ready-to-use column, prepacked with precharged Ni Sepharose 6 Fast Flow for preparative purification of histidine-tagged recombinant proteins by immobilized metal ion affinity chromatography (IMAC). | |
| Polypropylene | |
| 6% Cross-linked Agarose | |
| 5 bar (0.5 MPa, 70 psi) | |
| Pre-packed Column |
Veiligheid en behandeling
Signaalwoord
Gevarencategorie
Gevarenaanduiding
Veiligheidsmaatregel
Aanvullende informatie
- Waarschuwing
Gevarencategorie
- Ontvlambare vloeistof Categorie 3
Gevarenaanduiding
- H226-Ontvlambare vloeistof en damp.
- EUH208-Bevat . Kan een allergische reactie veroorzaken.
Veiligheidsmaatregel
- P280-Beschermende handschoenen/beschermende kleding/oogbescherming/gelaatsbescherming dragen.
- P210-Verwijderd houden van warmte, hete oppervlakken, vonken, open vuur en andere ontstekingsbronnen. Niet roken.
- P241-Explosieveilige elektrische/ventilatie-/verlichtings-/ apparatuur gebruiken.
- P303+P361+P353-BIJ CONTACT MET DE HUID (of het haar): verontreinigde kleding onmiddellijk uittrekken. Huid met water afspoelen/afdouchen.
- P235-Koel bewaren.
- P501-Inhoud/verpakking afvoeren naar .
Aanvullende informatie
- MIXTURE LIST-Bevat: ethanol, Nickel
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