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Cytiva HiScreen™ Blue, Ni and DEAE Sepharose™ Prepacked Fast Flow Columns

Used for method optimization and parameter screening

Merk:  Cytiva 28-9782-44


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Produktcode. 12305683

  • 204.00 /

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Beschrijving

Beschrijving

  • Based on 6% highly cross-linked agarose with an average bead size of 90μm
  • Processed flow properties and high loading capacities
  • Bed height of 10cm is designed to allow method optimization and parameter screening
  • Easily connected in series to achieve 20cm bed height
  • Small bed volume gives fast results and minimal sample/buffer consumption
  • Combines high capacity with high flow velocity and low backpressure to reduce process cycle times and increase productivity
  • Reproducible results, scalable to BioProcess™ columns packed with the same chromatography media using the same linear fluid velocity

  • Bed volume: 4.7mL
  • Bed dimension: 7.7 × 100mm
  • Matrix: 6% cross-linked agarose beads
  • Average particle size: 90μm
  • Flow velocity: <600cm/hr or <450cm/hr
  • Material (Column hardware and column tube): polypropylene
  • Column I.D.: 7.7mm

Blue Sepharose

  • Prepacked with BioProcess chromatography medium Blue Sepharose 6 Fast Flow
  • Purification of albumin, enzymes (including NAD+ and NADP+), coagulation factors, interferons and related proteins
Ni Sepharose
  • Prepacked with Nickel Sepharose 6 Fast Flow
  • Purification of histidine-tagged proteins with Immobilized Metal ion Affinity Chromatography (IMAC)
DEAE Sepharose
  • Prepacked with DEAE Sepharose Fast Flow
  • Weak anion exchanger (diethylaminoethyl group) BioProcess™ medium

TRUSTED_SUSTAINABILITY
Specificatie

Specificatie

0.1M HCl, 0.1M NaOH, 8M Urea
4.7 mL
7.7 mm
3.93 in.
<450 cm/hr.
Ni Sepharose
3 to 12
6% Cross-linked Agarose
Prepacked Fast Flow Column
HiScreen Ni Sepharose 6 FF column
0.3 in.
1 x 4.7mL
100mm
Polypropylene
1.5 bar
45 to 165μm
4° to 30°C
Purification of His-tagged Proteins with immobilized Metal ion affinity chromato graphy
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Signaalwoord
  • Waarschuwing

Gevarencategorie
  • Ontvlambare vloeistof Categorie 3

Gevarenaanduiding
  • H226-Ontvlambare vloeistof en damp.

Veiligheidsmaatregel
  • P210-Verwijderd houden van warmte, hete oppervlakken, vonken, open vuur en andere ontstekingsbronnen. Niet roken.
  • P501b-Inhoud/verpakking afvoeren naar verwerken volgens de plaatselijke/regionale/nationale/internationale voorschriften.

Aanvullende informatie
  • MIXTURE LIST-Bevat: ethanol, nickel
  • UNKNOWN ACUTE-Het mengsel bestaat voor 16.5 procent uit een of meer bestanddelen waarvan de acute toxiciteit niet bekend is.

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