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Cytiva HiLoad™ 16/600 Superdex™ 200 pg
Use for separating MAbs from dimers and contaminants. Cytiva HiLoad™ 16/600 Superdex™ 200 pg is ideal for high-resolution preparative gel filtration chromatography.
Merk: Cytiva 28-9893-35
Inclusief:
- Dismantling tool
- Support screen
- Net ring (10μm)
- O-ring
- Stop plugs
- Instructions
Beschrijving
Superdex prep grade (pg) is produced by covalent bonding of dextran to highly cross-linked agarose. Dextran component determined the separation properties of these media. Steep selectivity curves give gppd resolving power for peptides and proteins in the molecular weight range Mr 10 000 600 000 (Superdex 200 pg).- Used for separating MAbs from dimers and contaminants
- Short run times with high recovery
- Medium combines high mechanical strength with high hydrophilicity, allowing high flow rates, and minimal nonspecific interactions
- Separations can be scaled up to production levels
- 0.06 in. fittings to connect to AKTA design systems
- Theoretical plates: >13000m-1
- Flow rate: <1.6mL/min.
- Pressure max. (over the packed bed during operation): 3 bar [0.3MPa] (42psi)
- Media
- Average particle size: 34μm
- Matrix: composite of cross-linked agarose and dextran
- pH Stability working range: 3 to 12
- pH Stability cleaning range: 2 to 14
- Fractionation [Mr] globular proteins: 1 × 104-6 × 105
- Fractionation [Mr] dextrans: 1 × 103-1 × 105
- Shelf life from manufacture date: 5 years
- Chemical stability: all commonly used buffers, 1M NaOH, 0.1M HCl, 1M acetic acid, 8M urea, 6M guanidine HCl, 1% SDS, 70% ethanol, 30% iso-propanol, 30% acetonitrile
- Column
- Column I.D.: 0.62 in. (16mm)
- Inlet I.D.: 0.031 in. (0.8mm)
- Outlet I.D.: 0.031 in. (0.8mm)
- Chemical stability: resistant to aqueous solutions and solvents used in liquid chromatography. Not resistant to Acetone, Ketones, Chlorinated Hydrocarbons, Aliphatic Esters and Phenol
- Column hardware made from borosilicate glass, polyamide, fuororubber, Ethylene Propylene Diene (EPDM), ETFE, Superpolyoxymethylene
- Column tube made from borosilicate glass
- Bed support made from Polypropylene (PP) (reinforced glass fiber), Polyamide (PA)
- Porosity: 10μm
Should be avoided: >10% NaOH, >10% HCl, >5% acetic acid, and strong mineral acids
Informatie over bestelling
HiLoad columns are prepacked XK columns; Bed volume: 120mL; Bed dimensions: 0.62 x 23.62 in. (16 x 600mm); Sample volume: 5mL
Specificatie
| <5 mL | |
| 4°C to 30°C, 20% Ethanol |
Veiligheid en behandeling
Productidentificatie
Signaalwoord
Gevarencategorie
Gevarenaanduiding
Veiligheidsmaatregel
Aanvullende informatie
- HiLoad 16/600 Superdex 200 pg
Signaalwoord
- Waarschuwing
Gevarencategorie
- Ontvlambare vloeistof Categorie 3
Gevarenaanduiding
- H226-Ontvlambare vloeistof en damp.
Veiligheidsmaatregel
- P280-Beschermende handschoenen/beschermende kleding/oogbescherming/gelaatsbescherming dragen.
- P210-Verwijderd houden van warmte, hete oppervlakken, vonken, open vuur en andere ontstekingsbronnen. Niet roken.
- P241-Explosieveilige elektrische/ventilatie-/verlichtings-/ apparatuur gebruiken.
- P303+P361+P353-BIJ CONTACT MET DE HUID (of het haar): verontreinigde kleding onmiddellijk uittrekken. Huid met water afspoelen/afdouchen.
- P235-Koel bewaren.
- P501b-Inhoud/verpakking afvoeren naar verwerken volgens de plaatselijke/regionale/nationale/internationale voorschriften.
Aanvullende informatie
- MIXTURE LIST-Bevat: ethanol
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