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Cytiva First-Strand cDNA-synthesekit

For synthesis of single-stranded cDNA from mRNA templates prior to amplification by PCR

Merk:  Cytiva 27-9261-01

Produktcode. 10218334

  • 540.00 / 1 kit

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Inclusief: Bulk first-strand cDNA reaction mixes (Cloned murine reverse transcriptase, RNase inhibitor, RNase/DNase-free BSA, dATP, dCTP, dGTP, and dTTP in aqueous buffer), mRNA standard, DTT solution, RNase-free water, pd(N)6 primer, and Not I-(dT)19 bifunctional primer
This item is not returnable. View return policy

Beschrijving

Beschrijving

  • Preassembled bulk reaction mixes save time and minimize potential for pipetting errors
  • Primers are not included in the preassembled reaction mixes, allowing use of a primer of choice
  • Two primers are included with the kit
    • pd(N)6 primer (random hexamer) is used to prepare libraries for screening with antibodies, to increase the representation of 5'-ends of mRNAs having significant secondary structure, or to copy mRNAs lacking a poly(A) tail
    • Not I-(dT)18 bifunctional primer can be used to prime selectively on mRNA having a poly(A) tail and can also be used as a PCR primer following first-strand synthesis
    • The Not I restriction site, located at the 5'-end of the primer, is particularly useful for subsequent directional cloning of cDNA and PCR products

TRUSTED_SUSTAINABILITY
Specificatie

Specificatie

-15°C to -30°C
Voor synthese van enkelstrengs-cDNA uit mRNA-sjablonen vóór amplificatie door PCR
55
55 Reactions
Designed to generate full-length first-strand cDNA from mRNA templates. Following first-strand cDNA synthesis, the sample can be used directly for in vitro amplification using PCR or for second-strand synthesis using the Gubler-Hoffman method.
Bulk-first Strand cDNA-reactiemengsels, mRNA-standaard, DTT-oplossing, RNase-vrij water, pd(N)6-primer en NotI-(dT)18 bifunctionele primer
First-Strand cDNA Synthesis Kit
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Veiligheid en behandeling

Veiligheid en behandeling

Productidentificatie
  • First-Strand cDNA Synthesis Kit, 55 reactions

Gevarenaanduiding
  • EUH210-Veiligheidsinformatieblad op verzoek verkrijgbaar.

Aanvullende informatie
  • UNKNOWN ACUTE-Het mengsel bestaat voor 3 procent uit een of meer bestanddelen waarvan de acute toxiciteit niet bekend is.
  • UNKNOWN AQUATIC-Bevat 25.5% bestanddelen waarvan het gevaar voor het aquatisch milieu niet bekend is.

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