missing translation for 'onlineSavingsMsg'
Learn More
Learn More
Thermo Scientific™ RevertAid H zonder omgekeerde transcriptase
Thermo Scientific RevertAid H Minus Reverse Transcriptase is a recombinant M-MuLV RT. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity, but lacks RNase H activity due to a point mutation in the RNase H domain.
201.00 - 766.00
Spécification
| Content And Storage | • RevertAid H Minus Reverse Transcriptase (200 U/μL) |
|---|---|
| Indeling | Stand-alone Enzyme |
| Reaction Speed | 60 min. |
| Techniek | Reverse Transcription |
| Optimal Reaction Temperature | 42°C to 45°C |
| Code produit | Marque | Aantal | Prix | Quantité et disponibilité | |||||
|---|---|---|---|---|---|---|---|---|---|
| Code produit | Marque | Aantal | Prix | Quantité et disponibilité | |||||
|
10121360
|
Thermo Scientific™
EP0451 |
10,000 Units |
201.00
|
Please sign in to purchase this item. Need a web account? Register with us today! | |||||
|
11541515
|
Thermo Scientific™
EP0452 |
5 x 10,000 Units |
766.00
|
Please sign in to purchase this item. Need a web account? Register with us today! | |||||
Description
Thermo Scientific RevertAid H Minus Reverse Transcriptase is a recombinant M-MuLV RT. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity, but lacks RNase H activity due to a point mutation in the RNase H domain. RevertAid H Minus Reverse Transcriptase does not degrade RNA in RNA-DNA hybrids during synthesis of the first strand cDNA, and therefore high yields of full-length cDNA from long templates are obtained. RevertAid H Minus Reverse Transcriptase maintains activity over a wide temperature range (42–50°C).
Features of RevertAid H Minus Reverse Transcriptase include:
• High yields of full-length first strand cDNA up to 13 kb
• Optimum activity at 42–45°C
• Active up to 55°C
• Incorporates modified nucleotides (e.g., Cy3-, Cy5-, rhodamine-, aminoallyl-, and fluorescein-labeled nucleotides)
Applications
• First strand cDNA synthesis for RT-PCR and real-time RT-PCR
• Reverse transcription at elevated temperatures to reduce effects of secondary structure
• Synthesis of cDNA for cloning and expression
• Generation of labeled cDNA probes for microarrays
• DNA labeling
• Analysis of RNA by primer extension
Spécification
• RevertAid H Minus Reverse Transcriptase (200 U/μL) |
|
| 60 min. | |
| 42°C to 45°C | |
| Reduced | |
| 200 U/μL | |
| Separate components | |
| Up to 13 kb |
| Stand-alone Enzyme | |
| Reverse Transcription | |
| RevertAid H Minus | |
| Dry Ice | |
| First-Strand cDNA | |
| Reverse Transcription | |
| RNA |
For Research Use Only. Not for use in diagnostic procedures.
Vous avez repéré une opportunité d'amélioration ?Partager une correction de contenu
Correction du contenu d'un produit
Veuillez fournir vos retours sur le contenu du produit en remplissant le formulaire ci-dessous.
Nom du produit