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Thermo Scientific™ X-Gal-oplossing, gebruiksklaar

Effectively screen colonies with X-Gal, an inert chromogenic substrate for beta-galactosidase which hydrolyzes X-Gal, forming an intense blue precipitate.

Merk:  Thermo Scientific™ R0941


Produktcode. 10508750

  • 161.00 / 10 ml

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Beschrijving

Beschrijving

Formula: C14H15BrClNO6 Molecular weight: 408.6

X-Gal (5-bromo-4-chloro-3-indolyl-beta-D-galacto-pyranoside) is an inert chromogenic substrate for beta-galactosidase which hydrolyzes X-Gal into colorless galactose and 4-chloro-3-brom-indigo, forming an intense blue precipitate. Induction of the lacZ gene with IPTG leads to the hydrolysis of X-Gal and to the development of blue colonies (see Supporting data).

X-Gal Solution, ready-to-use, is stable, 0.22 μm membrane filtered solution formulated for direct use in conjunction with IPTG for blue/white colony screening.

Induction of the lacZ gene with IPTG leads to the hydrolysis of X-Gal and to the development of blue colonies. X-Gal Solution, ready-to-use, is a stable, 0.22μm membrane filtered solution formulated for direct use in conjunction with IPTG for blue/white colony screening.

Recommended for:

Blue/white colony screening to distinguish recombinant (white) from non-recombinant (blue) colonies (1); Visualization of beta-galactosidase reporter gene expression in transfected eukaryotic cells; Detection of beta-galactosidase activity in immunological and histochemical procedures.

Note:

Preparation of a 20mg/mL stock solution in dimethylformamide (DMF) or dimethylsulfoxide (DMSO) is recommended for X-Gal. DMF dissolves some plastic materials. The direct addition of DMF-containing solution to plastic Petri dishes should be avoided. DMF is toxic. Allow X-Gal solution containing dimethylformamide to completely dry before plating bacteria on agar plates.

TRUSTED_SUSTAINABILITY
Specificatie

Specificatie

Colorimetric
10 mL
Beta-Gal Substrate, X-Gal
X-Gal Solution
Chemical Substrate
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Veiligheid en behandeling

Veiligheid en behandeling

Productidentificatie
  • X-Gal Solution, ready-to-use

Signaalwoord
  • Waarschuwing

Gevarencategorie
  • Ontvlambare vloeistof Categorie 3
  • Sensibilisering van de huid Categorie 1

Gevarenaanduiding
  • H226-Ontvlambare vloeistof en damp.
  • H317-Kan een allergische huidreactie veroorzaken.

Veiligheidsmaatregel
  • P210-Verwijderd houden van warmte, hete oppervlakken, vonken, open vuur en andere ontstekingsbronnen. Niet roken.
  • P233-In goed gesloten verpakking bewaren.
  • P241-Explosieveilige elektrische/ventilatie-/verlichtings-/ apparatuur gebruiken.
  • P261-Inademing van stof/rook/gas/nevel/damp/spuitnevel vermijden.
  • P272-Verontreinigde werkkleding mag de werkruimte niet verlaten.
  • P280-Beschermende handschoenen/beschermende kleding/oog­bescherming/gelaatsbescherming dragen.
  • P302+P352-BIJ CONTACT MET DE HUID: met veel water/ wassen.
  • P303+P361+P353-BIJ CONTACT MET DE HUID (of het haar): verontreinigde kleding onmiddellijk uittrekken. Huid met water afspoelen/afdouchen.
  • P333+P313-Bij huidirritatie of uitslag: een arts raadplegen.
  • P362+P364-Verontreinigde kleding uittrekken en wassen alvorens deze opnieuw te gebruiken.
  • P370+P378-In geval van brand: blussen met .
  • P403+P235-Op een goed geventileerde plaats bewaren. Koel bewaren.
  • P501b-Inhoud/verpakking afvoeren naar verwerken volgens de plaatselijke/regionale/nationale/internationale voorschriften.

Aanvullende informatie
  • MIXTURE LIST-Bevat: ethanol, hydroquinone, DMSO

SDS
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For Research Use Only. Not for use in diagnostic procedures.