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Thermo Scientific™ GeneJET™ Miniprep-kit plasmide

Thermo Scientific GeneJET Plasmid Miniprep Kit utilizes an exclusive silica-based membrane technology in the form of a convenient spin column.

Merk:  Thermo Scientific™ K0502


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Produktcode. 12793184

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Beschrijving

Beschrijving

GeneJET Plasmid Miniprep Kit utilizes an exclusive silica-based membrane technology in the form of a convenient spin column. The kit recovers up to 20 μg of high copy plasmid DNA per isolation procedure.

Thermo Scientific GeneJET Plasmid Miniprep Kit utilizes an exclusive silica-based membrane technology in the form of a convenient spin column. The kit recovers up to 20 μg of high copy plasmid DNA per isolation procedure.

Highlights
• Efficient—high yields of up to 20 μg of high quality plasmid DNA
• Fast—procedure takes less than 14 minutes
• Convenient—no phenol/chloroform extraction or alcohol precipitation required
• Pure—purified DNA is immediately ready to use

Applications
• Fast isolation of high purity plasmid DNA suitable for all conventional molecular biology procedures, including:
• FastDigest or conventional restriction digestion
• Automated fluorescent and radioactive sequencing
• PCR
• In vitro transcription
• Transformation

Principle
A bacterial culture is harvested and lysed. The lysate is then cleared by centrifugation and applied on the silica column to selectively bind DNA molecules at a high salt concentration. The adsorbed DNA is washed to remove contaminants, and the pure plasmid DNA is eluted in a small volume of elution buffer or water. The purified DNA is ready for immediate use in all molecular biology procedures, such as fast and conventional digestion with restriction enzymes, PCR, in vitro transcription, transformation, and automated sequencing. Components Resuspension Solution Lysis Solution Neutralization Solution Wash Solution (concentrated) RNase A Solution Elution Buffer Spin Columns Collection Tubes Detailed Protocol

TRUSTED_SUSTAINABILITY
Specificatie

Specificatie

Spin Column
Silica Column
Bacteria
Compatible (Vacuum manifold)
GeneJET
50 Preps
<14 min
Spin Column
Mini
PCR, Cloning, Sequencing, Transformation, Nucleic Acid Labeling, In Vitro Transcription
< 100 μg (Small-Scale) Plasmid DNA
Plasmid MiniPrep Kit
Plasmid DNA
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Veiligheid en behandeling

Veiligheid en behandeling

Productidentificatie
  • GeneJET Plasmid Miniprep Kit

Signaalwoord
  • Gevaar

Gevarencategorie
  • Ernstig oogletsel/oogirritatie Categorie 2
  • Huidcorrosie/-irritatie Indeling Categorie 2
  • Acute toxiciteit Categorie 4

Gevarenaanduiding
  • H319-Veroorzaakt ernstige oogirritatie.
  • H315-Veroorzaakt huidirritatie.
  • H302-Schadelijk bij inslikken.

Veiligheidsmaatregel
  • P280-Beschermende handschoenen/beschermende kleding/oog­bescherming/gelaatsbescherming dragen.
  • P301+P312-NA INSLIKKEN: bij onwel voelen een ANTIGIFCENTRUM / arts/ raadplegen.
  • P303+P361+P353-BIJ CONTACT MET DE HUID (of het haar): verontreinigde kleding onmiddellijk uittrekken. Huid met water afspoelen/afdouchen.
  • P304+P340-NA INADEMING: de persoon in de frisse lucht brengen en ervoor zorgen dat deze gemakkelijk kan ademen.
  • P332+P313-Bij huidirritatie: een arts raadplegen.
  • P305+P351+P338-BIJ CONTACT MET DE OGEN: voorzichtig afspoelen met water gedurende een aantal minuten; contactlenzen verwijderen, indien mogelijk; blijven spoelen.
  • P337+P313-Bij aanhoudende oogirritatie: een arts raadplegen.
  • P302+P352-BIJ CONTACT MET DE HUID: met veel water/ wassen.
  • P264-Na het werken met dit product grondig wassen.
  • P373-NIET blussen wanneer het vuur de ontplofbare stoffen bereikt.

Aanvullende informatie
  • MIXTURE LIST-Bevat: Sodium lauryl sulfate, Sodium hydroxide,Guanidine hydrochloride

SDS
Documentatie

Documentatie

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For Research Use Only. Not for use in diagnostic procedures.