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Thermo Scientific™ GeneJET™ Miniprep-kit plasmide

Thermo Scientific GeneJET Plasmid Miniprep Kit utilizes an exclusive silica-based membrane technology in the form of a convenient spin column.

Merk:  Thermo Scientific™ K0502


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Produktcode. 10319699

  • 92.00 /

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Beschrijving

Beschrijving

GeneJET Plasmid Miniprep Kit utilizes an exclusive silica-based membrane technology in the form of a convenient spin column. The kit recovers up to 20 μg of high copy plasmid DNA per isolation procedure.

Thermo Scientific GeneJET Plasmid Miniprep Kit utilizes an exclusive silica-based membrane technology in the form of a convenient spin column. The kit recovers up to 20 μg of high copy plasmid DNA per isolation procedure.

Highlights
Efficient—high yields of up to 20 μg of high quality plasmid DNA
Fast—procedure takes less than 14 minutes
Convenient—no phenol/chloroform extraction or alcohol precipitation required
Pure—purified DNA is immediately ready to use

Applications
• Fast isolation of high purity plasmid DNA suitable for all conventional molecular biology procedures, including:
• FastDigest or conventional restriction digestion
• Automated fluorescent and radioactive sequencing
• PCR
In vitro transcription
• Transformation

Principle
A bacterial culture is harvested and lysed. The lysate is then cleared by centrifugation and applied on the silica column to selectively bind DNA molecules at a high salt concentration. The adsorbed DNA is washed to remove contaminants, and the pure plasmid DNA is eluted in a small volume of elution buffer or water. The purified DNA is ready for immediate use in all molecular biology procedures, such as fast and conventional digestion with restriction enzymes, PCR, in vitro transcription, transformation, and automated sequencing. Components Resuspension Solution Lysis Solution Neutralization Solution Wash Solution (concentrated) RNase A Solution Elution Buffer Spin Columns Collection Tubes Detailed Protocol

TRUSTED_SUSTAINABILITY
Specificatie

Specificatie

Spin Column
Silica Column
Bacteria
Compatible (Vacuum manifold)
GeneJET
50 Preps
<14 min
Spin Column
Mini
PCR, Cloning, Sequencing, Transformation, Nucleic Acid Labeling, In Vitro Transcription
< 100 μg (Small-Scale) Plasmid DNA
Plasmid MiniPrep Kit
Plasmid DNA
Product Suggestions

Product Suggestions

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Veiligheid en behandeling

Veiligheid en behandeling

Productidentificatie
  • GeneJET Plasmid Miniprep Kit

Signaalwoord
  • Waarschuwing

Gevarencategorie
  • Acute toxiciteit Categorie 4
  • Ernstig oogletsel/oogirritatie Categorie 2
  • Huidcorrosie/-irritatie Indeling Categorie 2

Gevarenaanduiding
  • H302-Schadelijk bij inslikken.
  • H315-Veroorzaakt huidirritatie.
  • H319-Veroorzaakt ernstige oogirritatie.

Veiligheidsmaatregel
  • P264-Na het werken met dit product grondig wassen.
  • P270-Niet eten, drinken of roken tijdens het gebruik van dit product.
  • P280-Beschermende handschoenen/beschermende kleding/oog­bescherming/gelaatsbescherming dragen.
  • P301+P310-NA INSLIKKEN: onmiddellijk een ANTIGIFCENTRUM/arts/ raadplegen.
  • P302+P352-BIJ CONTACT MET DE HUID: met veel water/ wassen.
  • P305+P351+P338-BIJ CONTACT MET DE OGEN: voorzichtig afspoelen met water gedurende een aantal minuten; contactlenzen verwijderen, indien mogelijk; blijven spoelen.
  • P330-De mond spoelen.
  • P332+P313-Bij huidirritatie: een arts raadplegen.
  • P337+P313-Bij aanhoudende oogirritatie: een arts raadplegen.
  • P362+P364-Verontreinigde kleding uittrekken en wassen alvorens deze opnieuw te gebruiken.
  • P501b-Inhoud/verpakking afvoeren naar verwerken volgens de plaatselijke/regionale/nationale/internationale voorschriften.

Aanvullende informatie
  • MIXTURE LIST-Bevat: guanidine hydrochloride

SDS
Documentatie

Documentatie

Certificates
Speciale aanbiedingen

Speciale aanbiedingen

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For Research Use Only. Not for use in diagnostic procedures.