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Thermo Scientific™ GeneJET™ PCR-zuiveringskit

Thermo Scientific GeneJET PCR Purification Kit utilizes a proprietary silica-based membrane technology in the form of a convenient spin column, eliminating the need for tedious resin manipulations or toxic phenol-chloroform extractions.

Merk:  Thermo Scientific™ K0701


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Produktcode. 10400450

  • 105.00 /

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Beschrijving

Beschrijving

Thermo Scientific GeneJET PCR Purification Kit utilizes a proprietary silica-based membrane technology in the form of a convenient spin column, eliminating the need for tedious resin manipulations or toxic phenol-chloroform extractions. It effectively removes primers, dNTPs, unincorporated labeled nucleotides, enzymes, and salts from PCR and other reaction mixtures.

The kit can be used for purification of DNA fragments from 25 bp to 20 kb with recovery rates up to 100%. Each GeneJET purification column has a total binding capacity of up to 25 μg of DNA, and the entire procedure takes 5 minutes. The purified DNA can be used in common downstream applications, such as sequencing, restriction digestion, labeling, ligation, cloning, in vitro transcription, blotting or in situ hybridization.

Highlights

  • Highly efficient: 90 to 100% recoveries in the range of 100 bp to 10 kb
  • Fast: procedure takes only 5 minutes
  • Convenient: spin columns are capped and assembled with collection tubes

Applications

Fast and efficient purification of DNA fragments ideal for use in all conventional molecular biology procedures including:

  • FastDigest or conventional restriction digestion
  • Automated fluorescent or radioactive sequencing
  • PCR
  • In vitro transcription
  • In situ hybridization
  • Labeling
  • Cloning
TRUSTED_SUSTAINABILITY
Specificatie

Specificatie

For better long-term performance, it is recommended to store the purification columns at 2°C to 8°C.
Not High-throughput Compatible (Manual)
PCR Purification Kit
5 min.
Real Time PCR (qPCR), Cloning, DNA Sequencing
dsDNA
Spin Column
GeneJET
50 Preps
DNA from Enzymatic Reactions (e.g. PCR)
Spin Column
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Veiligheid en behandeling

Veiligheid en behandeling

Productidentificatie
  • genejet PCR purif

Signaalwoord
  • Gevaar

Gevarencategorie
  • Acute toxiciteit Categorie 4
  • AQUATISCH GEVAAR OP LANGE TERMIJN Categorie 3 voor chronische toxiciteit
  • Huidcorrosie/-irritatie Indeling Categorie 1C

Gevarenaanduiding
  • H302-Schadelijk bij inslikken.
  • H314-Veroorzaakt ernstige brandwonden en oogletsel.
  • H412-Schadelijk voor in het water levende organismen, met langdurige gevolgen.

Veiligheidsmaatregel
  • P264-Na het werken met dit product grondig wassen.
  • P270-Niet eten, drinken of roken tijdens het gebruik van dit product.
  • P273-Voorkom lozing in het milieu.
  • P280-Beschermende handschoenen/beschermende kleding/oog­bescherming/gelaatsbescherming dragen.
  • P301+P310-NA INSLIKKEN: onmiddellijk een ANTIGIFCENTRUM/arts/ raadplegen.
  • P303+P361+P353-BIJ CONTACT MET DE HUID (of het haar): verontreinigde kleding onmiddellijk uittrekken. Huid met water afspoelen/afdouchen.
  • P305+P351+P338-BIJ CONTACT MET DE OGEN: voorzichtig afspoelen met water gedurende een aantal minuten; contactlenzen verwijderen, indien mogelijk; blijven spoelen.
  • P330-De mond spoelen.
  • P501b-Inhoud/verpakking afvoeren naar verwerken volgens de plaatselijke/regionale/nationale/internationale voorschriften.

Aanvullende informatie
  • MIXTURE LIST-Bevat: thiocyanic acid

SDS
Documentatie

Documentatie

Certificates
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For Research Use Only. Not for use in diagnostic procedures.