missing translation for 'onlineSavingsMsg'
Learn More

Afdrukken

Thermo Scientific™ FastDigest MspI

Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer.

Merk: Thermo Scientific™ FD0544

50.40 EUR valid until 2025-04-18
Use promo code "24107" to get your promotional price.

Produktcode. 10630461

72.00 / 400 reacties

Please sign in to purchase this item. Need a web account? Register with us today!

This item is not returnable. View return policy

Misschien heeft u interesse in deze alternatieven

Produktcode. 10334330

Thermo Scientific™ X5 MSPI (HPAII) 10U / UL 3000U

247.00 / 5 stuks

Produktcode. 15661932

Invitrogen™ 4000UNIT ANZA 65 MSPI

107.00 / 4000 eenheden

Beschrijving

Lambda DNA digested with MspI, 1.4% agarose, 328 cleavage sites

FastDigest enzymes are an advanced line of fast restriction enzymes that are all 100% active in the universal FastDigest and FastDigest Green reaction buffers.

The universal buffer allows rapid single-, double- or multiple DNA digestion within 5-15 minutes eliminating any need for buffer change or subsequent DNA clean-up steps. DNA modifying enzymes, such as Klenow Fragment, T4 DNA Ligase, alkaline phosphatases and T4 DNA Polymerase all have 100% activity in FastDigest Buffer. Therefore, enzymes for downstream applications can be directly added to the FastDigest reaction mix.

For additional convenience FastDigest Green Buffer includes a density reagent and two tracking dyes for direct loading of digestion reaction products on gels.

Short incubation times and optimal composition of the universal FastDigest Buffer eliminate star activity effects.

The universal buffer allows rapid single-, double-, or multiple DNA digestion within 5–15 minutes eliminating any need for buffer change or subsequent DNA clean-up steps. See Reaction Conditions for FastDigest Enzymes for a table of enzyme activity, heat inactivation and incubation times for this and other FastDigest restriction enzymes. DNA modifying enzymes, such as Klenow Fragment (Cat. No. EP0051), T4 DNA Ligase (Cat. No. EL0014), alkaline phosphatases and T4 DNA Polymerase (Cat. No. EP0061) all have 100% activity in FastDigest Buffer. Therefore, enzymes for downstream applications can be directly added to the FastDigest reaction mix.

For additional convenience FastDigest Green Buffer includes a density reagent and two tracking dyes for direct loading of digestion reaction products on gels.

Short incubation times and optimal composition of the universal FastDigest Buffer eliminate star activity effects.

Features
• 100% activity of all FastDigest enzymes in the universal buffer
• 100% buffer compatibility with downstream applications
• Complete digestion in 5–15 minutes
• Direct loading on gels
• No star activity
• 176 FastDigest enzymes available

Applications
• Molecular cloning
• Restriction site mapping
• Genotyping
• Southern Blot
• Restriction fragment length polymorphism (RFLP)
• SNP analysis

Note: The FastDigest Green Buffer offers the same high performance in DNA digestion and downstream applications as the colorless FastDigest Buffer. For applications that require product analysis by fluorescence excitation (e.g. concentration measurements in UV light) the colorless FastDigest Buffer is recommended.

For methylation sensitivity, refer to product specifications.

Meer weergeven

Specificatie

Content And Storage	• FastDigest MspI (400 μL) • 10X FastDigest Buffer (1.0 mL) • 10X FastDigest Green Buffer (1.0 mL) Store at –20°C.
Enzyme	MspI
Sensitive to Heat Inactivation	No
Type IIS RE	No
Producttype	Restriction Enzyme
Product Line	FastDigest™

Methylation Sensitivity	Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive, Not CpG Methylation-Sensitive
Compatible Buffer	10X FastDigest Buffer/FastDigest Green Buffer
Optimal Reaction Temperature	37°C
Aantal	400 μL (400 Reactions)
Research Category	Traditional Cloning
Overhang	5' protruding ends

Product Suggestions Documentatie

Documentatie

Product Information

Manuals

User Guide: Reaction Set-up for Digestion of Multiple DNA Samples Versie: Jan. 2015 Bestandsgrootte: 71.4 kB

User Guide: Scaling up DNA Digestion Reaction Versie: Jan. 2015 Bestandsgrootte: 60.9 kB

User Guide: FastDigest MspI Versie: Jan. 2015 Bestandsgrootte: 52 kB

User Guide: Double and Multiple Digestion of DNA Versie: Jan. 2015 Bestandsgrootte: 71.3 kB

User Guide: Fast Digestion of DNA Versie: Jan. 2015 Bestandsgrootte: 67.9 kB

Scientific Resources

Reference Materials

LabAid: FastDigest restriction enzymes Bestandsgrootte: 62.2 kB

Certificates

Certificaten

A lot number is required to show results for certificates. To find your lot number on previous orders use our order status area.

Lot Number

5 results found

Lot Number	Certificate Type	Date	Catalog Number
Lot Number2820419	Certificate TypeCertificate of Analysis	Date29/01/2025	Catalog NumberFD0544
Lot Number2874420	Certificate TypeCertificate of Analysis	Date28/01/2025	Catalog NumberFD0544
Lot Number2960567	Certificate TypeCertificate of Analysis	Date26/01/2025	Catalog NumberFD0544
Lot Number3015720	Certificate TypeCertificate of Analysis	Date26/01/2025	Catalog NumberFD0544
Lot Number3059955	Certificate TypeCertificate of Analysis	Date25/01/2025	Catalog NumberFD0544

Product Content Correction

Your input is important to us. Please complete this form to provide feedback related to the content on this product.

Product Title

Thermo Scientific™ FastDigest MspI > 400μL

Your Name

Email Address *

Type of Issue *

Detailed Description of Issue/Request *

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Cancel Submit

Thank You! Your feedback has been submitted. Fisher Scientific is always working to improve our content for you. We appreciate your feedback.

For Research Use Only. Not for use in diagnostic procedures.

Spot an opportunity for improvement?Share a Content Correction