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Thermo Scientific™ 50X TAE-buffer (Tris-acetaat-EDTA)

Optimize electrophoresis of both genomic and large supercoiled DNA in agarose and polyacrylamide gels with Thermo Scientific™ 50X TAE Buffer (Tris-acetate-EDTA).

Merk:  Thermo Scientific™ B49

65.49 EUR valid until 2025-04-18
Use promo code "24107" to get your promotional price.


Produktcode. 10399519

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Beschrijving

Beschrijving

Use the Thermo Scientific 50X TAE Buffer (Tris-acetate-EDTA) for electrophoresis of nucleic acids in agarose and polyacrylamide gels. You can use this buffer for both genomic and large supercoiled DNA, and you can also use this as both a running and a gel preparation buffer.

Usage Recommendations

  • Use fresh 1X TAE both for the gel and for the electrophoresis run
  • To prepare 1X TAE buffer add 20ml of 50X TAE buffer to 980ml of deionized water and mix well

Quality Control

  • The absence of endo-, exodeoxyribonucleases and ribonucleases confirmed by appropriate quality tests

1X Composition

  • 40mM Tris
  • 20mM acetic acid
  • 1mM EDTA
  • pHof 50X TAE: 8.4

Recommended for:

Electrophoresis of nucleic acids in agarose and polyacrylamide gels; Used both as a running buffer and as a gel preparation buffer; Filtered through a 0.22μm membrane; Recommended for resolution of RNA and DNA fragments l.

Note:

TAE buffer has a relatively low buffering capacity, therefore periodic replacement of the buffer during prolonged electrophoresis is recommended.

TRUSTED_SUSTAINABILITY
Specificatie

Specificatie

50X
1 L
TAE Buffer
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Productidentificatie
  • TAE Buffer (Tris-acetate-EDTA) (50X)

Signaalwoord
  • Waarschuwing

Gevarencategorie
  • Dzia anie toksyczne na narzady docelowe Categorie 2

Gevarenaanduiding
  • H373-Kan schade aan organen.

Veiligheidsmaatregel
  • P260-Stof/rook/gas/nevel/damp/spuitnevel niet inademen.
  • P314-Bij onwel voelen een arts raadplegen.
  • P501b-Inhoud/verpakking afvoeren naar verwerken volgens de plaatselijke/regionale/nationale/internationale voorschriften.

Aanvullende informatie
  • MIXTURE LIST-Bevat: acetic acid

SDS
Documentatie

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For Research Use Only. Not for use in diagnostic procedures.