TaqMan™ TaqMan™ Gene Expression Master Mix

Beschrijving

TaqMan™ Gene Expression Master Mix has been validated with TaqMan™ Gene Expression Assays and Applied Biosystems™ real-time systems, providing precise quantification for a variety of gene expression-based real-time qPCR applications, including rare transcript detection, gene expression analysis, pathogen detection and viral load quantification,co-amplification of two targets, as well as specific detection of gene family members

TaqMan™ Gene Expression Master Mix contains AmpliTaq Gold™ DNA Polymerase, UP (Ultra Pure) for hot start activation and improved detection of bacterial targets, a blend of dNTPs with dTTP/dUTP and Uracil-DNA Glycosylase (UDG) to minimize carry-over PCR contamination, and a passive internal reference based on proprietary ROX™ dye for superb precision on Applied Biosystems™ real-time PCR instruments.

• Provides precise and reliable real-time qPCR quantification over nine logs of linear dynamic range
• TaqMan Gene Expression Master Mix is an optimized 2X mix that contains all of the components, excluding the template and primers, for sensitive detection down to one copy of target
• Extended benchtop stability provides consistent results for automated liquid-handling systems
• This makes it the choice for high-throughput sample processing overnight or over the weekend
• Validated with TaqMan gene expression assays and Applied Biosystems real-time systems, providing precise quantification for a variety of gene expression–based real-time qPCR applications
• Applications include rare transcript detection, gene expression analysis, pathogen detection and viral load quantification, co-amplification of two targets, as well as specific detection of gene family members
• Store at 2°C to 8°C

TaqMan Gene Expression Master Mix contains:

• AmpliTaq Gold DNA Polymerase, UP (Ultra Pure), for hot start activation and improved detection of bacterial targets
• A blend of dNTPs with dTTP/dUTP and uracil-DNA glycosylase (UDG) to minimize carry-over PCR contamination
• A passive internal reference based on proprietary ROX dye for superb precision on Applied Biosystems real-time PCR instruments

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