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Cytiva Amersham™ AlkPhos Direct™ ECF Detection Module

Fast and convenient systems for all high-sensitivity applications using DNA, RNA or oligonucleotide probes

Merk:  Cytiva RPN3685

Produktcode. 10208724

  • 748.00 /

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Beschrijving

Beschrijving

AlkPhos Direct Labeling and Detection Systems are based on the rapid, direct labeling of DNA or RNA probes with thermostable alkaline phosphatase. CDP-Star™ or ECF™ detection can be used. DNA or RNA probes are labeled during a 30-minute reaction and resulting probes can be used without purification. Hybridization stringency can be controlled by temperature as well as salt concentration.

  • Hybridization stringency can be controlled by elevating temperatures up to 75°C
  • Detection of 60 fg target DNA (single-copy gene) in a genomic Southern blot
  • ECF detection for quantitation experiments
  • Rapid protocol saves two to three hours over conventional indirect methods

Informatie over bestelling

Each system include the following reagents, sufficient for 25 or 50 labeling reactions and detection of 2500cm2: labeling reagent, crosslinker, control DNA, reaction buffer, hybridization buffer, blocking agent, ECF detection reagents.

TRUSTED_SUSTAINABILITY
Specificatie

Specificatie

2500cm2 membrane
Sufficient for 25 or 50 labeling reactions and detection of 2500cm2 membrane: labeling reagent, crosslinker, control DNA, reaction buffer, hybridization buffer, blocking agent, ECF detection reagents.
Each
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  • Ernstig oogletsel/oogirritatie Categorie 1
  • Huidcorrosie/-irritatie Indeling Categorie 2
  • Dzia anie toksyczne na narzady docelowe Categorie 2

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