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Invitrogen™ MagMAX™-96 Total RNA Isolation Kit
The MagMAX-96 Total RNA Isolation Kit is designed for the rapid, moderate-throughput purification of RNA from animal and plant cells and tissue.
Merk: Invitrogen™ AM1830
510.00 EUR valid until 2025-03-28
Use promo code "24107" to get your promotional price.
Inclusief: Kit contents: 1 Processing Plate and Lid; 11mL Lysis/Binding Solution Concentrate; 18mL Wash Solution 1 Concentrate; 55mL Wash Solution 2 Concentrate; 12mL RNA Rebinding Concentrate; 10mL Elution Buffer; 6mL MagMAX TURBO DNase Buffer; 1.1mL RNA Binding Beads; 1.1mL Lysis/Binding Enhancer; 110μL TURBO DNase. Contains enough reagents to isolate RNA from 96 samples.
Beschrijving
The MagMAX-96 Total RNA Isolation Kit is designed for the rapid, moderate-throughput purification of RNA from animal and plant cells and tissue. RNA purified using the kit is ideal for PCR and RT-PCR applications. The kit uses magnetic bead–based purification and includes sufficient reagents for 96 reactions.
Benefits of MagMAX magnetic bead–based purification
Magnetic beads offer many benefits compared to other technologies for isolating RNA. Beads bind RNA more efficiently than glass fiber filters, resulting in higher and more consistent RNA yields. Additionally, because filters are not used, there is no risk of filter clogging due to cellular particulates in samples. Since only a small volume of magnetic beads is needed for high-efficiency binding, the bound RNA can be eluted in just 20–50 μL of nuclease-free water, concentrating RNA from large, dilute samples.
Accommodates diverse sample types
The kit accommodates diverse biological samples and a broad range of cell and tissue input amounts. The kit is optimized for high-throughput isolation of RNA from 25 cells to 2 x 106 cells, as well as small plant and mammalian tissue samples (up to 10mg). The protocol is fully amenable to automation. Detailed guidelines for general automation are included with the kit.
- Enhanced lysis/binding conditions to accommodate larger sample inputs
- Reagents for TURBO DNase™ treatment included to minimize DNA contamination and preserve RNA integrity
- Includes protocol for RNA isolation from plant tissues
- 96 reactions per kit suitable for moderate throughput
- Automated protocols, high-throughput compatible users
Accessory products
Lysis/Binding Solution Concentrate, Wash Solution 1 Concentrate, and Wash Solution 2 Concentrate are components of the kit that may also be purchased separately. The Plant RNA Isolation Aid can be used in conjunction with the MagMAX-96 Total RNA Isolation Kit for the isolation of RNA from plant tissues.
Specificatie
| • 1 Processing Plate; room temperature • 11 mL Lysis/Binding Solution (concentrated); room temperature • 18 mL Wash Solution 1 (concentrated); room temperature • 55 mL Wash Solution 2 (concentrated); room temperature • 12 mL RNA Rebinding (concentrated); room temperature • 10 mL Elution Buffer; room temperature • 6 mL MagMAX TURBO DNase Buffer; room temperature • 1.1 mL RNA Binding Beads; 4°C • 1.1 mL Lysis/Binding Enhancer; -20°C • 110 μL TURBO DNase; -20°C |
|
| 50 μL | |
| Total RNA | |
| KingFisher™ Duo Prime, KingFisher™ Flex, Automated Liquid Handling Systems | |
| 96 Preps | |
| Cells: ≤106 Plant: ≤10 mg Tissue: ≤5 mg |
| Magnetic Bead | |
| Cells, Plants, Tissue | |
| High-throughput Compatible | |
| RT-PCR, qPCR, cDNA library construction, NGS, microarray analysis, blot hybridization, Northern blotting, in vitro translation, nuclease protection assays, nucleic acid labeling | |
| Box 1: Dry Ice Box 2: Room Temperature |
|
| ≤20 pg per cell |
Veiligheid en behandeling
Productidentificatie
Signaalwoord
Gevarencategorie
Gevarenaanduiding
Veiligheidsmaatregel
Aanvullende informatie
- MagMAX-96 Total RNA Isolation Kit
Signaalwoord
- Gevaar
Gevarencategorie
- Acute toxiciteit Categorie 4
- AQUATISCH GEVAAR OP LANGE TERMIJN Categorie 3 voor chronische toxiciteit
- Ernstig oogletsel/oogirritatie Categorie 2
- Sensibilisatie van de luchtwegen Categorie 1
- Huidcorrosie/-irritatie Indeling Categorie 1C
- Huidcorrosie/-irritatie Indeling Categorie 2
Gevarenaanduiding
- H302-Schadelijk bij inslikken.
- H314-Veroorzaakt ernstige brandwonden en oogletsel.
- H315-Veroorzaakt huidirritatie.
- H319-Veroorzaakt ernstige oogirritatie.
- H334-Kan bij inademing allergie- of astmasymptomen of ademhalingsmoeilijkheden veroorzaken.
- H412-Schadelijk voor in het water levende organismen, met langdurige gevolgen.
Veiligheidsmaatregel
- P260-Stof/rook/gas/nevel/damp/spuitnevel niet inademen.
- P261-Inademing van stof/rook/gas/nevel/damp/spuitnevel vermijden.
- P264-Na het werken met dit product grondig wassen.
- P270-Niet eten, drinken of roken tijdens het gebruik van dit product.
- P273-Voorkom lozing in het milieu.
- P280-Beschermende handschoenen/beschermende kleding/oogbescherming/gelaatsbescherming dragen.
- P284-¢Bij ontoereikende ventilatie! adembescherming dragen.
- P301+P310-NA INSLIKKEN: onmiddellijk een ANTIGIFCENTRUM/arts/ raadplegen.
- P301+P312-NA INSLIKKEN: bij onwel voelen een ANTIGIFCENTRUM / arts/ raadplegen.
- P301+P330+P331-NA INSLIKKEN: de mond spoelen - GEEN braken opwekken.
- P302+P352-BIJ CONTACT MET DE HUID: met veel water/ wassen.
- P303+P361+P353-BIJ CONTACT MET DE HUID (of het haar): verontreinigde kleding onmiddellijk uittrekken. Huid met water afspoelen/afdouchen.
- P304+P340-NA INADEMING: de persoon in de frisse lucht brengen en ervoor zorgen dat deze gemakkelijk kan ademen.
- P305+P351+P338-BIJ CONTACT MET DE OGEN: voorzichtig afspoelen met water gedurende een aantal minuten; contactlenzen verwijderen, indien mogelijk; blijven spoelen.
- P310-Onmiddellijk een ANTIGIFCENTRUM/arts/ raadplegen.
- P330-De mond spoelen.
- P342+P311-Bij ademhalingssymptomen: Een ANTIGIFCENTRUM/arts/ raadplegen.
- P363-Verontreinigde kleding wassen alvorens deze opnieuw te gebruiken.
- P501b-Inhoud/verpakking afvoeren naar verwerken volgens de plaatselijke/regionale/nationale/internationale voorschriften.
Aanvullende informatie
- MIXTURE LIST-Bevat: Proteinase, tritirachium album serine, Thiocyanic acid
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Product Title
For Research Use Only. Not for use in diagnostic procedures.
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