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Thermo Scientific™ 1-Step™ TMB-Blotting substraatoplossing

Ready-to-use blue TMB peroxidase (HRP) substrate specially formulated for chromogenic detection in Western blot and IHC experiments.

Merk:  Thermo Scientific™ 34018


Produktcode. 10066433

  • 203.00 / 250 ml

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Beschrijving

Beschrijving

Thermo Scientific™ Pierce TMB-Blotting 1-Step Solution is a ready-to-use solution of the blue TMB peroxidase (HRP) substrate that is specially formulated for chromogenic detection in Western blotting and immunohistochemistry methods.

TMB (3,3',5,5'-tetramethylbenzidine; MW = 240.4) is most often used as a soluble substrate to detect the activity of peroxidase-conjugated antibodies in ELISA. Pierce TMB-Blotting Solution is formulated to precipitate and localize the blue reaction product onto membrane surfaces or tissue samples at sites where the peroxidase-substrate reaction occurs. TMB-Blotting Substrate produces a very large signal-to-noise ratio, although its high sensitivity requires careful washing and blocking to avoid background staining. When performing immunohistochemical techniques, aqueous counterstains and mounting medium must be used.

Highlights:

  • HRP substrate – for sensitive detection of horseradish peroxidase activity on solid media

  • Chromogenic – no special equipment needed for visualization; produces dark blue bands in Western blots; is not as susceptible to fading as AEC or 4-CN substrates

  • Ready-to-use – no organic solvents are required to dissolve; no dilution necessary for use

  • Complete – does not require other buffers or addition of hydrogen peroxide

TRUSTED_SUSTAINABILITY
Specificatie

Specificatie

Substrate
Liquid
250 mL
Horseradish Peroxidase
Colorimetric
1-Step™
Horseradish Peroxidase
Upon receipt store product at 4°C. Product is shipped at ambient temperature.
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Veiligheid en behandeling

Veiligheid en behandeling

Productidentificatie
  • 1-Step TMB-Blotting, 250 mL

Signaalwoord
  • Waarschuwing

Gevarencategorie
  • Acute toxiciteit Categorie 4
  • Dzia anie toksyczne na narzady docelowe Categorie 2

Gevarenaanduiding
  • H302-Schadelijk bij inslikken.
  • H371-Kan schade aan organen.

Veiligheidsmaatregel
  • P270-Niet eten, drinken of roken tijdens het gebruik van dit product.
  • P260-Stof/rook/gas/nevel/damp/spuitnevel niet inademen.
  • P264-Na het werken met dit product grondig wassen.
  • P301+P312-NA INSLIKKEN: bij onwel voelen een ANTIGIFCENTRUM / arts/ raadplegen.
  • P330-De mond spoelen.
  • P308+P311-NA (mogelijke) blootstelling: Een ANTIGIFCENTRUM/arts/ raadplegen.

Aanvullende informatie
  • MIXTURE LIST-Bevat: Methyl alcohol

SDS
Documentatie

Documentatie

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For Research Use Only. Not for use in diagnostic procedures.